Identification and characterization of a prokaryotic Mannosyl-oligosaccharide Glucosidase (MOGS) and establishment of a functional complementation assay for MOGS activity

Mannosyl-oligosaccharide Glucosidase (MOGS) initiates glycan trimming by specifically cleaving α-1,2-linked glucose residues on glycoproteins. MOGS is a key enzyme for proper N-glycosylation, and its defects are associated with rare disease MOGS related congenital disorders of glycosylation (MOGS-CDG). Although MOGS has been identified from most of eukaryotic organisms, its existence in prokaryotic remains unclear.