Activity of α1,6-fucosyltransferase (FUT8) is reduced by depletion of oligosaccharyltransferase subunits

Alpha-1,6-fucosyltransferase (FUT8) transfers fucose to the innermost GlcNAc residue of N-glycans, forming the core fucose structure. Core fucose critically regulates the functions of various glycoproteins and is associated with several diseases, including chronic obstructive pulmonary disease and cancer. However, the regulatory mechanisms of its enzymatic activity and the intracellular localization of FUT8 remain largely unknown. […] In this study, we assessed the enzymatic activity of FUT8 with knockdown of each OST subunit and showed that silencing ribophorin II (RPN2) as well as RPN1 significantly reduced FUT8 activity.